The Dothan Area Chamber of Commerce was founded in 1919.

In his book, Dothan, A Pictorial History, author Wendell Stepp notes that in the early 1930s, the Chamber was little more than an office to establish prices for cotton and other produce.

A new era dawned for the Chamber in 1934 when Harry Hall, president of the Rotary Club, and Theodore Jackson, president of the Kiwanis Club formed a joint committee from the two clubs. Two goals emerged: establish a meaningful chamber of commerce and cut the weeds consuming downtown. The modernized Chamber of Commerce made its first home on the second floor of City Hall (Dothan Opera House) at 115 North St. Andrews Street. Since then Chamber has moved several times:

• 1954 - 1967 130 North Oates Street
• 1967 - 1989, 300 North Foster Street
• 1989 - 2003, 440 Honeysuckle Road

As of July 1, 2003, 102 Jamestown Boulevard became the permanent home of the Dothan Area Chamber of Commerce.